The second step in the machinery is much more efficient
in extracting energy. Instead of being reduced to lactate or ethanol,
pyruvate enters the citric acid cycle, where it is broken
down to C02, with hydrogen atoms being used to reduce
NAD+ and FAD to NADH and FADH2. Some additional
ATP also is made along the way. The NADH and FADH2 from
the citric acid cycle, plus the NADH from fermentation, which now
is not needed to convert pyruvate into something else, all flow
into the third process, the respiratory chain. Here they
are reoxidized to NAD+ and FAD and are recycled. The
hydrogen atoms ultimately are added to 02 to make water,
and the free energy that is liberated is stored in the form of ATP.
The overall process - the combustion of glucose with oxygen - is
carried out in a series of small steps so that the maximum amount
of energy from the reaction can be saved.
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